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disinfection methods

 Types of Disinfectant and Methods


1. Alcohol

2. Surface active agent

3. Aldehyde

4. Dyes

5. Halogens

6. Phenols

7. Gases 

8. Metallic salts

9. Fumigation

10. Hydrogen peroxide

1. Alcohol;

a. Ethyl alcohol

Used in skin antiseptic.


b. Isopropyl alcohol

Used for disinfection of clinical thermometer.

For example; savlon 

c. Methyl alcohol

Effective against fungal spores.

2. Aldehyde

1. Formaldehyde

It is bactericidal, sporicidal

Preserve anatomical specimens

Destroy Anthrax spores from wool

Used to Sterilized and clean instrument

Formaldehyde gas used for sterilizing instrument.

2. Glutaraldehyde

Effective against tubercle bacilli, fungi and virus.

Used for Sterilization of cystoscope and bronchoscope.

3. Use to Sterilized;

1. Anesthesia tube

2. Face mask

3. Metal instruments

3. Dye

1. Aniline dyes 

2. Acridine dyes;

Used extensively as skin and wound antiseptics.

Used in clinical medicine 

Disinfect the clinical air

Kill or destroy the reproductive capacity of microbial cell.

4. Halogens

1. Iodine

Used as disinfectant.

For example; Betadine

Act against microbial spores

Destroy tubercle bacilli and virus

Used for cleaning or disinfecting skin and treatment of injuries.


2. Chlorine

Disinfectants

Bactericidal

Against viruses

Water supplies, swimming pools

Diary industry use chlorine for disinfection

Used as antiseptics for dressing wound (Chloramines).

5. Phenols (carbolic acid)

These are coaltar carbolic acid compound.

Uses of phenols;

1. Phenolic compound use as disinfectant

2. Used to kill microorganisms

3. Powerful microbicidal substance

4. Lysol and cresol (compound) widely used as disinfectant in hospital.

5. Used in control of pyrogenic cocci in surgical and neonatal units in hospital.


6. Gases

1. Ethylene oxide

Used for sterilizing heart-lung machines, respirators, sutures and dental equipments.

Used to sterilized glass, metal, paper surfaces, clothing, plastic etc.


2. Formaldehyde gas

Used to fumigation OT

3. Beta propiolactone BPL

Killing microorganism

Bactericidal 

7. Surface active agent

1. Anionic

2. Cationic

3. Nonionic

4. Amphoteric

Used as wetting agents.

8. Metallic salts

They are protein coagulants hence kill the microorganism.

1. Salt of Silver

Used in burns.

2. Salt of copper

Used as fungicides 

3. Salts of mercury

Used as mild antiseptic


9. Lower temperature gas plasma (LTGP)

This leads to generation of free radicals which destroy the microorganism.

10. Ozone Sterilization

Use as disinfectant for water and food.

It is used both in gas and liquid form as an antimicrobial agent for treatment, stroage and processing of foods including meat, poultry and eggs.

Characteristics of an Ideal Disinfectant

It should;

1. High penetration power

2. Non toxic and non irritant

3. Not stain tissue

4. Not have unpleasant ordor

5. Easily available

6. Able to destroy infectious agent

7. Long shelf life

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