methods of identifying microorganisms

Laboratory method in identifying microorganisms 

Microbiologist use 5 basic procedures to examine and characterize microbes;

1. Inoculation

2. Incubation

3. Isolation

4. Inspection (Observation)

5. Identification 

1. Aseptic technique in laboratory preparation and analysis.

Sterilization

Disinfection

Sanitation

2. Culture Technique

Types of culture media

Incubation and isolation

3. Fixation and Staining

1. Negative and simple strain

2. Differential strain

3. Special strains

4. Fixation and Staining for electron microscopy

4. Identification Technique

Morphology; shape, size

Culture characteristics; growing medium

Physiological/ Biochemical characteristics

Molecular analysis