Agglutination Reaction
When a insoluble antigen is mixed with its antibody at suitable temperature (37 degree Celsius and PH; 7.4, the particles are clumped or agglutinated.
In presence of electrolytes (NaCl).
Technique of Agglutination Test
1. Slide agglutination test
2. Tube agglutination test
1. Slide agglutination test (micro agglutination)
It is qualitative test.
Widely used method to detect unknown antigen.
(Bacteria, RBCs in blood grouping)
On a clear glass slide a drop of antigen (made in saline) is mixed with a drop of antiserum.
The reaction occurs immediately.
Clumps are formed in few minutes.
2. Tube agglutination test
Quantitative test
Macro agglutination
Quantitative test used to determine or estimate the titer of antibody and to confirm the result of slide agglutination test.
It is used for serological diagnosis of typhoid, paratyphoid, brucellosis and typhus fever.
For example; Widal test
0 Comments