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autoclave method

Steam under pressure/ temperature above 100 degree Celsius/Autoclave

Method by steam under pressure is most widely used. Effective and most reliable method of Sterilization. It has more penetrating power.

It destroy microorganisms including their spores.


Working of Autoclave

Sterilization by steam under pressure is generally carried at; 

Temperature in between 108- 147 degree Celsius.


a. Pressure; 15lb

b. Time for 25-20 minutes

c. At temperature 121degree celcius

Uses;

By using appropriate temperature, pressure and time. Materials can be Sterilized;

a. Dressing and instrument

b. Laboratory ware and media

c. Pharmaceutical product

Steam Sterilizers (Autoclaves) in use;

a. Medical lab; sterile medical, surgical equipment 

b. Laboratory autoclaves; glassware 

c. Bowl and instrument Sterilizers

d. Rapid cooling Sterilizers

e. Decontaminate specific biological waste before disposable 

f. Used for Sterilization of culture media.

Plastic tube, pipette tips, plastic container 

g. Hospital dressing Sterilizers (108degree celcius)

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