Difference between of cell wall of Gram Positive and gram Negative bacteria
Character gram positive Gram negative
1. Thickness Thicker Thinner
2. Periplasmic space Absent Present
3. Lipids. Absent or small Present
4. Teichoic acid Present. Absent
5. Peptidoglycan 16-80nm thick multilayer. 2nm thin, single layer
6. Staining Gram positive cells stain purple during gram staining. Gram negative cells stain pink during gram staining.
7. Outer membrane. Absent Present
8. Periplasmic space. Absent. Present
9. Resistance against antibiotics. Less resistance to antibiotics. More resistance to antibiotics.
10. Cell wall Cell wall is smooth Cell wall is wavy.
11. Porin. Absent. Present in outer membrane
12. Lipopolysaccharide. Usually not found. High
13. Mesosome. Mesosome is more prominent. Mesosome is less prominent
14. Flagella structure. 2 ring in basal body. 4 ring in basal body
15. Toxins produce. Primarily exotoxins retain crystal 4 ring in basal body primarily
Violet dye and stain dark violet or. endotoxins. It can be
purple coloured, They remain decolourized to accept
coloured. Stain.
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