Role of Hot Oven in Sterilization
Hot oven is physical method of dry heat sterilization. Working on dry heat.
Sterilization is process of removing or destroying all microorganisms include virus, bacteria and their spores from article or surface without destroying it's quality and quantity.
Uses of Hot Air Oven
Sterilization of article at high temperature.
For example;
Equipment include-
Glassware
Forceps
Scissors
Scalpels
Pharmaceutical products
Glass
Syringes
Test tube
Pipettes
Powders
Its work on principle of conduction where heat is absorbed by exterior surface of item then passed towards next layer.
Modes of action - protein denaturation
Factors influence sterilization by heat
1. Dry or moist
2. Temperature and time
3. Number of microorganisms
4. Microorganism type
Temperature
Extremely high temperature.
170 degree celcius for 30 minutes.
160 degree celsius for 60 minutes.
150 degree celsius for 150 minutes.
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