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Pseudomonas (gram negative bacilli)

 Pseudomonas

Cause Nosocomial Infection.

It is involved in hospital acquired infection.

These are live freely in water, soil, plants and air contaminated equipments.

Morphology

Gram negative bacilli actively motile, non capsulated, non sporing.

Size; 1.5 - 3 micrometer X 0.5 micrometre

Characteristics

Pseudomonas is a strict aerobe.

Grow wall on ordinary media nutrient broth and nutrient agar.

Sources and Modes of Transmission

Direct contact

Contact with wound or burn

Widely spread in hospital environment

Present in water, soil and dust air.

Pathogenesis

Causes fever shock.

Localized infection, Nosocomial Infection.

Due to resistance to common antibiotic and antiseptic widely in hospital.

It causes localized and systemic illness.

1. Localized illness

Wound and burn infection

Chronic otitis media

Urinary tract infection 

Eye Infection

Diarrhea

Respiratory tract infection

2. Systemic Infection

Bacteremia

Pneumonia

Bone and joint infection

Endocarditis

CNS infection; when meninges breached.

Skin/ soft tissue infection

Urinary tract and pelvic infection

Wound sepsis


Laboratory Diagnosis

Specimen collection

Sputum, blood, Urine, CSF sample 

Gram stain and morphology

Gram -ve bacilli, actively motile by polar flagella.

Cultural characteristics and culture

Strict aerobe media 

Biochemical test

Ferment glucose forming acid only.

Treatment and Prevention

1. Following universal safety precautions in health care setting.

2. After swimming must take off costume and showers properly.

3. Ears should be dried carefully

 Treatment

Combine anti- microbial therapy is administered.

Mainly use of antibiotic like; Gentamicin

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