Leptospira Interrogans
The pathogenic is slender, tightly coiled, culturable Spirochaete with a single, thin, axial filament and hooked ends.
Many serogroup and serotype are recognised.
Actively motile, they are best obsorved by electronic microscopy.
Sources and mode of Transmission
The disease is transmitted to humans when infected animal urine, contaminates water or food.
Pathogens can also enter in host body through skin abrasion or the conjunctiva.
Pathogenesis
Cause disease leptospirosis.
Jaunice, marsh fever, weil's disease and swincherd's disease.
Hemorrhage tissue nacrosis, septic meningitis.
Laboratory Diagnosis
Specimen collection
Urine, blood, CSF
Microscopic examination
Dark ground microscopy shows tightly coiled Spirochaetes with single,thin, axial filament and hooked ends.
Culture
Medium enrich with rabbit blood
Serum
Grow on chicken embryo
Animal is inoculated into culture media for isation of organisms.
Very useful method for diagnosis.
Cultured aerobic microaerophilic condition.
Optimum temperature to grow between 25-30 degree Celsius at PH 7.2-7.5
Serological test
1. Screening test
1. Complete fixation test
2. Haemagglutination test
3. ELISA
4. SEL
5. Indirect influorescent
Antibody test (widely used for detected of leptospira).
2. Serotype specific test
Treatment
Penicillin and tetracycline are effective for treatment.
0 Comments