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Treponema pallidum

 Treponema palladium

Causing Syphilis

Treponema species due to their motile nature, can be poorly stained with gram negative staining.

Treponema palladium is cause for disease syphilis.


Morphology

Gram -ve bacilli, long, flexible, spiral or cork screw shaped rods with endoflagella, actively motile.

Size; 10 micrometre long and 0.1- 0.2 micrometre wide.

Source and Mode of Transmission

It is mainly transmitted by-

1. Sexual contact

2. Infected blood transfusions

Therefore infected semen, vaginal secretion and blood is major source of infection.

Pathogenesis

Organism enter through sexual contact or transplacently.

It cannot exist or survive outside the host body for more than few minutes.

Organism penetrates in mucous membrane through genitalia and produce following disease;

Syphilis

Primary stage;

Hard genital or oral ulcer develop at site of inoculation.

Secondary stage;

Appearance of red rash, any part of body, palms of hand and sole of feet.

Tertiary stage;

Degeneration of nervous system, cardiovascular lesion.


Congenital Syphilis

Treponema palladium

Can transmited through placenta to fetus, cause abortion of fetus.

Other/ treponemal infection 


Laboratory Diagnosis

Specimen collection

Lesion infect area.

Dark field microscopic examination

Appear spiral, flexible, slow movement, black background appear.

It can also be confirmed by direct fluorescent antibody test 


DFA-TP test

Better and safe method for microscopic diagnosis of treponema palladium.

Serological test 

Infected with treponema palladium produce two types of antibodies;

1. Treponemal tests (Treponemes are used as antigen)

a. RPCF test (Reiter's protein complement fixation test)

b. TPIA test  (Treponema palladium immune adherence test)

c. TPA test ( Treponema palladium agglutination test)

d. FTA test (Fluorescent treponema palladium)

e. FTA- ABS test (Fluorescent treponemal antibody absorption test)

f. TPHA ( Treponema palladium haemagglutination assay test)

2. Non- treponemal tests (Cardiolipin or lipoidal antigen is used)

a. Wassermann test

b. Kahn test

c. VDRL (venereal diseases research loboratory test)


Treatment

One single treatment with penicillin is effective for primary and secondary syphilis. 

No antibiotic resistance has been reported.

Alternative therapy with erythromycin and tetracycline may also be effective.

Prevention

Treatment of pregnant mother with appropriate antibiotics prevent congenital Syphilis.

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