Laboratory Diagnosis
1. Direct microscopy
2. KOH (potassium hydroxide preparation)
Specimen placed in drop of 10% KOH on microscopic slide and cover with Coverslip.
It is heated gently and examine under microscope.
Yeasts cells and hyphae may be observed.
2. Gram staining
It is done to observe gram positive yeasts as in case of candida species.
3. India Ink preparation
India Ink preparation used for detection of capsulated yeast such as Cryptococcus neoformans in cerebrospinal fluid (CSF).
2. Culture
SDA (Sabouraud dextrose agar) solution is used and it is incubated at 25-37 degree Celsius for 3 weeks.
Chloramphenicol is added in culture medium to suppress growth of contaminated Bacteria.
While cycloheximide is incorporated to suppress contaminating fungi.
Microscopy is performed from fungal colony to study morphology of hyphae, spores and other structure.
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